BκF 对体外培养人肝细胞诱导细胞色素P450 1A 的研究

doi:10.3969/j.issn.1004-616x.2002.03.008

癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (3): 167-170.doi: 10.3969/j.issn.1004-616x.2002.03.008

BκF 对体外培养人肝细胞诱导细胞色素P450 1A 的研究

杨　慧1,刘　宁2,Laurance S.Kaminsky3

1. 江西医学院免疫学教研室, 江西　南昌　330006; 2. 江西医学院环境卫生学教研室,江西　南昌　330006; 3. New York State Department of Health, Wadsworth Center, Albany, New York 12201, USA

收稿日期:2001-07-10 修回日期:2001-12-30 出版日期:2002-07-30 发布日期:2002-07-30
通讯作者: 杨　慧

ANALYSIS OF CYTOCHROME P450 1A COMPOSITION IN HUMAN HEPATOCYTES INDUCED BY BκF

YANG Hui1, LIU Ning2, Laurance S.Kaminsky3

1. Department of Immunology, Jiangxi Medical College, Nanchang 330006, China; 2. Department of,Wadsworth Center, Albany , New York 12201, USA

Received:2001-07-10 Revised:2001-12-30 Online:2002-07-30 Published:2002-07-30
Contact: YANG Hui1

摘要/Abstract

摘要： 目的: 研究体外培养的人肝细胞经苯并[κ]萤蒽(BκF) 诱导后其细胞色素P4501A(CYP1A) 亚家族的组成。方法:采用 Bis2Tris2HCI 缓冲的聚丙烯酰胺凝胶免疫印迹电泳系统和多克隆羊抗人CYP1A1/ CYP1A2 及兔抗人CYP1A2 抗体对经5 μmol/ L BκF 诱导24 h 的6 名捐献者的肝细胞中表达的CYP1A1 和CYP1A2 进行分离鉴定。结果: 6 份样品中5 份仅检出 CYP1A1 ,而另1 份则2 种CYP 均被检出,其CYP1A1/ CYP1A2 的比值为0. 942 ;溶剂对照诱导的肝细胞CYP1A1 和CYP1A2 均未检出。结论:新鲜的人肝细胞经BκF 诱导后CYP1A 亚家族的表达因人而异,与直接从组织制备的人肝微粒体中的CYP1A 的水平有明显不同。

关键词: 细胞色素P450 1A1, 细胞色素P450 1A2, 人肝细胞, 多环芳烃

Abstract: Purpose : To explore the P450 (CYP) CYP1A subfamily composition in the f resh human hepatocyte cultures induced by benzo[κ] fluoranthene (BκF) . Methods : An immunoblot system with Bis2Tris2HCl buffered polyacrylamide gel which clearly resolves human CYP1A1 and CYP1A2 , polyclonal goat anti2human CYP1A1/ CYP1A2 and rabbit anti2human CYP1A2 antibodies were used to probe the expressed CYP1A1 and CYP1Acomposition in six individual human hepatocyte cultures induced with 5μmol/ L (BκF) for twenty2four hours. Results : CYP1A1 was found in five of the six cultures. Both CYP1A1 and 1A2 were simultaneously found in one of them at a ratio of CYP1A1/ CYP1A2 of 0. 942. There was neither CYP1A1 nor CYP1A2 in vehiclet reated hepatocyte cultures. Conclusion : The CYP1A expression in the f resh human hepatocyte cultures induced by BκF is individually variable and it s CYP1A level is different f rom that of human liver microsomal preparations.

Key words: cytochrome P450 1A1, cytochrome P450 1A2, human hepatocyte, polycyclic aromatic hydrocarbon

中图分类号:

R994. 3

杨　慧, 刘　宁, Laurance S.Kaminsky. BκF 对体外培养人肝细胞诱导细胞色素P450 1A 的研究[J]. 癌变·畸变·突变, 2002, 14(3): 167-170.

YANG Hui, LIU Ning, Laurance S.Kaminsky. ANALYSIS OF CYTOCHROME P450 1A COMPOSITION IN HUMAN HEPATOCYTES INDUCED BY BκF[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(3): 167-170.

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BκF 对体外培养人肝细胞诱导细胞色素P450 1A 的研究

ANALYSIS OF CYTOCHROME P450 1A COMPOSITION IN HUMAN HEPATOCYTES INDUCED BY BκF

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